cell tracker green cmfda  (Yeasen Biotechnology)

Journal: Advanced Healthcare Materials

Article Title: Fibrillar Bundles as Fibrous Filler Materials for Attaining Cell Anisotropy in Bioprinting

doi: 10.1002/adhm.202503767

Figure Lengend Snippet: Bioprinting composite bioinks containing encapsulated C2C12 myoblast cells in GelMA with 3.75% concentration. a) Schematic representation of GelMA composite bioink preparation. b) Quantification of the cell viability after 3D bioprinting of the composite bioink using live/dead assay and fluorescent microscopy imaging ( calcein AM (green) and ethidium homodimer‐1 (red)) with and without the fibrillar bundles. Fiber caps are spotted with red color due to the autofluorescence. Monitoring the cell behavior using green cell tracker‐stained myoblasts and fluorescence microscopy images (n=3), c) directly, and d) three days after 3D bioprinting. e) a larger snapshot of the fully cell‐covered bundle fiber cultured for three days after 3D bioprinting.

Article Snippet: Instead, Cell‐tracker green CMFDA was purchased from Invitrogen, Insulin‐transferrin‐selenium Solution (ITS), Fetal calf serum (FCS), horse serum (HS), penicillin−streptomycin (pen/strep), and GlutaMax were purchased from Gibco.

Techniques: Concentration Assay, Live Dead Assay, Microscopy, Imaging, Staining, Fluorescence, Cell Culture